 Wysłany: Śro 11:30, 30 Mar 2011 Temat postu: O-GlcNAc synthesis of peptides _1143 |
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| O-GlcNAc synthesis of peptides
Time, the role of TFA stable ,jimmy choo south africa, mass spectrometry did not see the results of glycosylation in the off peak . GlcNAc was obtained acetyl -protected glycopeptides crude , the use of high performance liquid chromatography (HPLC) to separate them . After the spin take acetonitrile , water use in the freeze-dried product removed. Finally , the use of sodium methoxide in methanol solution (pH to 9-10) on the GlcNAc glycopeptide hydroxyl protecting groups ( acetyl) off . After reaction with C- 18 packing small columns purification (acetonitrile / water gradient elution ), to get the final peptide O-GlcNAc modification of the product of R4, by electrospray ionization mass spectrometry (ESI-MS) identification. |
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