px215467
Weteran
Dołączył: 29 Lip 2010
Posty: 202
Przeczytał: 0 tematów
Ostrzeżeń: 0/5 Skąd: vmxxrv
|
Wysłany: Śro 11:30, 30 Mar 2011 Temat postu: O-GlcNAc synthesis of peptides _1143 |
|
|
O-GlcNAc synthesis of peptides
Time, the role of TFA stable ,[link widoczny dla zalogowanych], mass spectrometry did not see the results of glycosylation in the off peak . GlcNAc was obtained acetyl -protected glycopeptides crude , the use of high performance liquid chromatography (HPLC) to separate them . After the spin take acetonitrile , water use in the freeze-dried product removed. Finally , the use of sodium methoxide in methanol solution (pH to 9-10) on the GlcNAc glycopeptide hydroxyl protecting groups ( acetyl) off . After reaction with C- 18 packing small columns purification (acetonitrile / water gradient elution ), to get the final peptide O-GlcNAc modification of the product of R4, by electrospray ionization mass spectrometry (ESI-MS) identification.
Post został pochwalony 0 razy
|
|